mouse monoclonal anti 4 hydroxynonenal hne antibody  (R&D Systems)

Journal: Nature Communications

Article Title: Compression-induced NF-κB activation sustains tumor cell survival in confinement by detoxifying aldehydes and promotes metastasis

doi: 10.1038/s41467-025-67452-7

Figure Lengend Snippet: a–d IF analysis with anti-ALDH1B1, anti-RelA, and anti-CD31 antibodies was performed on lung cancer patients. Images from two representative patients showing tumor cells constrained or unconstrained in lung capillaries from n = 61 patients are shown ( a ). Semiquantitative scoring was performed ( b–d ) (paired t -test, two-tailed ( b , c ); (Pearson product moment correlation test, two-tailed ( d ); r = 0.78, P < 0.0001). e–g IF analysis with anti-ALDH1B1, anti-4-HNE, and anti-CD31 antibodies was performed on the same patients. Images from two representative patients showing tumor cells constrained or unconstrained in lung capillaries from n = 61 patients are shown ( e ). Semiquantitative scoring was performed ( f and g ) (paired t -test, two-tailed ( f ); (Pearson product moment correlation test, two-tailed ( g ); r = −0.60, P < 0.0001). h , i IF analysis with anti-ALDH1B1, anti-CD31 antibodies and TUNEL staining on the same patients. Images from two representative patients showing tumor cells constrained or unconstrained in lung capillaries from n = 61 patients are shown ( h ). Semiquantitative scoring was carried out ( i ) (Pearson product moment correlation test, two-tailed; r = −0.56, P < 0.0001). j , k Semiquantitative scoring of ALDH1B1 and 4-HNE intensity in tumor cells confined within capillaries of same patients was calculated. Patients from the cohort ( n = 61) were categorized based on metastatic recurrence status: with metastatic recurrence ( n = 29) and without metastatic recurrence ( n = 31). ALDH1B1 intensity was determined by averaging values from ( d , g , and i ), while 4-HNE intensity was derived from ( g ). Data represent the mean ± SD for the two groups (two-tailed Student’s t test). Source data are provided as a Source Data file.

Article Snippet: Rabbit polyclonal anti-ALDH1B1 (15560-1-AP, Proteintech Group); Rabbit polyclonal anti-CSK23 (PA5-98299, Thermo Fisher Scientific); Rabbit monoclonal anti-Flag (20543-1-AP, Proteintech Group); Rabbit monoclonal anti-HA (3724, Cell Signaling Technology); Mouse monoclonal anti-Actin (60008-1-lg, Proteintech Group); Mouse monoclonal anti-Tubulin (T9026, Sigma); Mouse IgG antibody (sc-2025, Santa Cruz Biotechnology); Rabbit monoclonal anti-RelA (8242, Cell Signaling Technology); Mouse monoclonal anti-RelA (66535-1-Ig, Proteintech Group); Rabbit monoclonal anti-IKKβ (8943, Cell Signaling Technology); Mouse monoclonal anti-IκBα S32/36 phosphorylation (9246, Cell Signaling Technology); Rabbit monoclonal anti-IKKβ S177/181 phosphorylation (2697, Cell Signaling Technology); Rabbit monoclonal anti-IκBα (10268-1-AP, Proteintech Group); Rabbit monoclonal anti-Lamin B1 (12586 s, Cell Signaling Technology); Goat polyclonal anti-CD31(AF3628, Novus Biologicals); Mouse monoclonal anti-4-HNE (MAB3249, R&D Systems); Goat-anti-mouse IgG second antibody (31160, Thermo Fisher Scientific); Goat-anti-rabbit IgG second antibody (31210, Thermo Fisher Scientific); Donkey-anti-mouse Alexa Fluor 488 (A21202, Thermo); Donkey-anti-rabbit Alexa Fluor cy3 (711-165-152, Jackson ImmunoResearch); Donkey-anti-goat Alexa Fluor 647 (A21447, Thermo).

Techniques: Two Tailed Test, TUNEL Assay, Staining, Derivative Assay